{"id":3255,"date":"2023-07-03T14:45:43","date_gmt":"2023-07-03T13:45:43","guid":{"rendered":"https:\/\/pebble.bio\/?post_type=news&#038;p=3255"},"modified":"2024-03-12T09:27:56","modified_gmt":"2024-03-12T09:27:56","slug":"dermal-tolerance","status":"publish","type":"news","link":"https:\/\/pebble.bio\/news\/dermal-tolerance\/","title":{"rendered":"The LIVING-LIMB system: An Ethical &#038; Reproducible Approach for Local Tolerance Dermatological Studies"},"content":{"rendered":"<p style=\"text-align: justify;\"><span class=\"x_ContentPasted0\"><strong>[28<sup class=\"x_ContentPasted0\">th<\/sup>\u00a0June 2023]<\/strong> &#8211; The advanced LIVING-LIMB System is a significant advancement for dermatological research. This validated next-generation ex-vivo perfusion system is designed to perform highly reproducible local (dermal) tolerance studies. The LIVING-LIMB system is an ethical, time-efficient, and cost-effective alternative to determine phototoxicity, cutaneous sensitisation, patch testing, dermal irritation, and injection site irritation in dermatological research.\u00a0<\/span><\/p>\n<p style=\"text-align: justify;\"><span class=\"x_ContentPasted0\">\u00a0<\/span><\/p>\n<div style=\"width: 1920px;\" class=\"wp-video\"><!--[if lt IE 9]><script>document.createElement('video');<\/script><![endif]-->\n<video class=\"wp-video-shortcode\" id=\"video-3255-1\" width=\"1920\" height=\"1080\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/pebble.bio\/wp-content\/uploads\/2023\/02\/15112022-technical-video-v8.mp4?_=1\" \/><a href=\"https:\/\/pebble.bio\/wp-content\/uploads\/2023\/02\/15112022-technical-video-v8.mp4\">https:\/\/pebble.bio\/wp-content\/uploads\/2023\/02\/15112022-technical-video-v8.mp4<\/a><\/video><\/div>\n<p>&nbsp;<\/p>\n<p style=\"text-align: justify;\"><b><span class=\"x_ContentPasted0\">Validation and Reproducibility<\/span><\/b><span class=\"x_ContentPasted0\">: The LIVING-LIMB System has undergone comprehensive optimisation and validation over 10 years, to establish its reliability and reproducibility. The system fully replicates the physiological conditions of living tissue by restoring blood flow to a perfused limb from a pig, while maintaining tissue viability. This has been validated through extensive assessment of physiological\u00a0<span data-markjs=\"true\" data-ogac=\"\" data-ogab=\"\" data-ogsc=\"\" data-ogsb=\"\"><span class=\"x_markegmtrbk4b x_ContentPasted0\">mark<\/span><\/span>ers, histological evaluations, and functional analyses. \u00a0<\/span><\/p>\n<p style=\"text-align: justify;\"><span class=\"x_ContentPasted0\">\u00a0<\/span><\/p>\n<p style=\"text-align: justify;\"><b><span class=\"x_ContentPasted0\">Ethical and Efficient Approach:\u00a0<\/span><\/b><span class=\"x_ContentPasted0\">One of the system&#8217;s advantages lies in its ethical approach. By employing a perfused limb from a pig, the LIVING-LIMB System obviates the need for live animal experimentation, aligning with 3Rs and providing a real alternative to in-vivo models.\u00a0Furthermore, biological material is retrieved via the food industry, meaning no laboratory animals are used.<b class=\"x_ContentPasted0\">\u00a0<\/b><\/span><\/p>\n<p style=\"text-align: justify;\"><span class=\"x_ContentPasted0\">\u00a0<\/span><\/p>\n<p style=\"text-align: justify;\"><span class=\"x_ContentPasted0\">Unlike conventional animal-based models, the system eliminates the necessity for protracted regulatory approvals or animal licenses. This streamlined approach enables scientists to start experiments within days, rather than months.\u00a0<\/span><\/p>\n<p style=\"text-align: justify;\"><span class=\"x_ContentPasted0\">The LIVING-LIMB system also significantly reduces costs by up to 90%, when compared to large animal experiments. Researchers can now allocate their resources more efficiently, fostering innovation and progress in the field of dermatological research.\u00a0<\/span><\/p>\n<p style=\"text-align: justify;\"><b><span class=\"x_ContentPasted0\">\u00a0<\/span><\/b><\/p>\n<p style=\"text-align: justify;\"><b><span class=\"x_ContentPasted0\">Applications in Local (Dermal) Tolerance Studies:\u00a0<\/span><\/b><span class=\"x_ContentPasted0\">The LIVING-LIMB System is optimised for dermal tolerance studies. Drugs and cosmetics can be applied topically, subcutaneously, or intra-muscularly (via injection) to the perfused limb, enabling evaluation of various facets of local tolerance.<b class=\"x_ContentPasted0\">\u00a0<\/b><\/span><\/p>\n<p style=\"text-align: justify;\"><span class=\"x_ContentPasted0\">\u00a0<\/span><\/p>\n<p style=\"text-align: justify;\"><span class=\"x_ContentPasted0\">By subjecting the perfused limb to controlled conditions and monitoring skin responses in real time, researchers can acquire precise and reproducible data to assess the safety and efficacy of substances. The LIVING-LIMB System enables accurate evaluation of adverse reactions, immune responses, and skin sensitisation potential, contributing to the development of safer and more effective drugs and cosmetics.\u00a0<\/span><\/p>\n<p style=\"text-align: justify;\"><span class=\"x_ContentPasted0\">\u00a0<\/span><\/p>\n<p style=\"text-align: justify;\"><b><span class=\"x_ContentPasted0\">Summary:\u00a0<\/span><\/b><span class=\"x_ContentPasted0\">The introduction of the LIVING-LIMB System\u00a0<span data-markjs=\"true\" data-ogac=\"\" data-ogab=\"\" data-ogsc=\"\" data-ogsb=\"\"><span class=\"x_markegmtrbk4b x_ContentPasted0\">mark<\/span><\/span>s a milestone in dermatological research, providing a validated and reproducible ex-vivo perfusion system for dermal tolerance studies, that is more translational compared to existing small animal approaches. This innovative system offers a reliable, cost-effective, ethical and efficient alternative to traditional animal-based models.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p class=\"x_MsoNormal\"><span class=\"x_ContentPasted1\">With the LIVING-LIMB system, you benefit from;\u00a0\u00a0<\/span><\/p>\n<ul class=\"x_ContentPasted1\" type=\"disc\">\n<li class=\"x_MsoNormal\"><b><span class=\"x_ContentPasted1\">Enhanced efficiency<\/span><\/b><span class=\"x_ContentPasted1\">: your R&amp;D process is streamlined, which saves you time and resources<\/span><\/li>\n<li class=\"x_MsoNormal\"><b><span class=\"x_ContentPasted1\">Iterative refinement:<\/span><\/b><span class=\"x_ContentPasted1\">\u00a0we identify and solve major issues early on to mitigate risk and delays\u00a0\u00a0<\/span><\/li>\n<li class=\"x_MsoNormal\"><b><span class=\"x_ContentPasted1\">Regulatory success:\u00a0<\/span><\/b><span class=\"x_ContentPasted1\">we know what regulatory authorities need so your data is tailored to meet their requirements, smoothing out that path to clinical trial approval<\/span><\/li>\n<li class=\"x_MsoNormal\"><b><span class=\"x_ContentPasted1\">Ethical:<\/span><\/b><span class=\"x_ContentPasted1\">\u00a0the LIVING-LIMB SYSTEM does not use laboratory animals \u2013 all biological material is surplus from the food industry.\u00a0\u00a0\u00a0<\/span><\/li>\n<li class=\"x_MsoNormal\"><b><span class=\"x_ContentPasted1\">Cost saving:<\/span><\/b><span class=\"x_ContentPasted1\">\u00a0the complete lack of laboratory animals significantly reduces costs \u2013 that\u2019s around an 80% cost-saving compared to large animal<i class=\"x_ContentPasted1\">\u00a0in vivo\u00a0<\/i>experiments<\/span><\/li>\n<li class=\"x_MsoNormal\"><b><span class=\"x_ContentPasted1\">Time saving:\u00a0<\/span><\/b><span class=\"x_ContentPasted1\">your study can be started in days and completed in weeks, rather than months or years<\/span><\/li>\n<li class=\"x_MsoNormal\"><b><span class=\"x_ContentPasted1\">Expertise:\u00a0<\/span><\/b><span class=\"x_ContentPasted1\">The Pebble team are key opinion leaders in the field, and we have performed over 1200 procedures to date.\u00a0<\/span><\/li>\n<\/ul>\n<div style=\"width: 1920px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-3255-2\" width=\"1920\" height=\"1080\" poster=\"https:\/\/pebble.bio\/wp-content\/uploads\/2023\/07\/PBL-Logo-and-Strapline-888.png\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"https:\/\/pebble.bio\/wp-content\/uploads\/2023\/07\/limb-video-3.mp4?_=2\" \/><a href=\"https:\/\/pebble.bio\/wp-content\/uploads\/2023\/07\/limb-video-3.mp4\">https:\/\/pebble.bio\/wp-content\/uploads\/2023\/07\/limb-video-3.mp4<\/a><\/video><\/div>\n","protected":false},"featured_media":7589,"template":"","meta":{"_acf_changed":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"site-sidebar-layout":"no-sidebar","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center 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